In vitro synthesis of subunits of bacterial luciferase in an Escherichia coli system.
نویسندگان
چکیده
RNA extracted from the luminescent bacterium Vibrio harveyi was translated in an Escherichia coli system. RNA from highly luminescent cells produced both alpha and beta subunits of luciferase in vitro, as confirmed by immunoprecipitation and partial proteolysis. RNA from cells before induction of luminescence did not direct luciferase synthesis.
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ورودعنوان ژورنال:
- Journal of bacteriology
دوره 153 1 شماره
صفحات -
تاریخ انتشار 1983